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Product Info Summary
| SKU: | LS003758 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript variant rom-k1 |
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Product info
Product Name
KCNJ1 (NM_000220) Human Over-expression Lysate
SKU/Catalog Number
LS003758
Size
100 µg
Description
Transient overexpression lysate of potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript variant rom-k1
Storage & Handling
Cite This Product
KCNJ1 (NM_000220) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003758)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KCNJ1 Human Over-expression Lysates NM_000220.
Western blot validation of overexpression lysate (LS003758) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNJ1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KCNJ1 (Source: Uniprot.org, NCBI)
Gene Name
KCNJ1
Full Name
ATP-sensitive inward rectifier potassium channel 1
Weight
44.795kDa
Superfamily
inward rectifier-type potassium channel (TC 1.A.2.1) family
Alternative Names
ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectifier potassium channel 1; inwardly rectifying K+ channel; KIR1.1; potassium inwardly-rectifying channel, subfamily J, member 1; ROMK1inwardly rectifying subfamily J member 1 KCNJ1 KIR1.1, ROMK, ROMK1 potassium inwardly rectifying channel subfamily J member 1 ATP-sensitive inward rectifier potassium channel 1|ATP-regulated potassium channel ROM-K|inward rectifier K(+) channel Kir1.1|inwardly rectifying K+ channel|potassium channel, inwardly rectifying subfamily J member 1|potassium voltage-gated channel subfamily J member 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KCNJ1, check out the KCNJ1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNJ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KCNJ1 (NM_000220) Human Over-expression Lysate (LS003758)
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No publications found for LS003758
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